MDM2 gene expression in nasopharyngeal carcinoma and its relationship with p53 protein expression and EB virus latent infection / 临床耳鼻咽喉头颈外科杂志

Objective:To define the expression levels of MDM2 gene in nasopharyngeal carcinoma (NPC)and its relationship with p53 protein expression and EB virus latent infection. Method :MDM2 gene expression atmRNA and protein levels,p53 protein and EB virus DNA were detected by nonradioactive in situ hybridization(ISH) ,immunohistochemistry(IHC) and polymerase chain reaction (PCR) separately in 46 cases of NPC tissuesand 12 cases of chronic inflammation of nasopharyngeal epithelium (CINE). Result: Fourteen cases of NPCshowed MDM2 mRNA and protein overexpression, 38 cases were p53 protein positive,and 43 cases were EBV-DNA positive. Neither MDM2 nor p53 protein was expressed in any case of CINE. MDM2 expression was signifi-cantly related to p53 protein expression ( P ＜0. 05), but not to EB virus latent infection in NPC. Conclusion:MDM2 gene may play an important role in the pathogenesis of NPC through interacting with p53 protein.

ELISA(Phadezym PRIST and RAST)of total IgE and allergen specific IgE in patients with perennial allergic Rhintis (A report of 54 cases) / 中国免疫学杂志

Total IgE in both serum and nasal secretion of 26 cases with perennial allergic rhinitis(PAR),14 cases with nonallergic rhinitis and 14 cases of healthy control was quantitatively measured by using Phadezym PRIST which is a solid phase enzyme immunossay based on sandwich technique.The geometric mean with standard deviation of total IgE in serum was significantly greater (P